文章：A New Producer of Echinocandins, Ascomycete Coleophoma sp. Isolated from the Lichen Stereocaulon paschale

發(fā)表單位：Russian Academy of Sciences

期刊：Microbiology

IF值：1.3    

中科院分區(qū)：Q4

檢測應(yīng)用：菌生長曲線

具體方法：To determine the effect of the extract on the growth kinetics of test cultures (Yarrowia lipolytica VKM Y-3322, Candida parapsilosis S134, Candida albicans T5 and T6), we used the method of cultivation in immunological 96-well plates using a multimodal reader Feyond-A300 (China).

文章：Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

發(fā)表單位：江南大學(xué)

期刊：Scientific Reports

IF值：3.8    

中科院分區(qū)：Q2

檢測類型：熒光

檢測應(yīng)用：鈣離子

具體方法：The spectrofluorometer (Feyond-A300, All Sheng Co. Ltd., Hangzhou, China) was used to assess the fluorescence of Fluo-4-Ca2+ at excitation at 488 nm and emission in the range of 512–520 nm. Data was documented at intervals of 10 s.

文章：The efficient activity of plant essential oils for inhibiting Botrytis cinerea and Penicillium expansum: Mechanistic insights into antifungal activity

發(fā)表單位：Universidad de La Frontera

期刊：Microbiological Research

IF值：6.1    

中科院分區(qū)：Q1

檢測類型：熒光

檢測應(yīng)用：真菌生物量與生物質(zhì)的干重

具體方法：The fluorescence intensity was measured in a multi-modal microplate reader (Feyond-a300,Allsheng). The arbitrary fluorescence units were corrected according to the absorbance at 450 nm, assuming that fungal biomass was correlated linearly with the dry weight of the biomass.

文章：Multivariate Modular Metabolic Engineering for High Titer Uridine Triphosphate Production in Escherichia coli

發(fā)表單位：江南大學(xué)

期刊：ACS Sustainable Chemistry & Engineering

IF值：7.1    

中科院分區(qū)：Q1

檢測類型：熒光

檢測應(yīng)用：GFP蛋白

具體方法：Samples were taken as required and were further diluted 1:5 in 200 μL of fresh LB with antibiotics and inducers for the measurement using a Feyond-A300 microplate reader (All Sheng Co. Ltd., Hangzhou, China). GFP abundance was quantified at an excitation wavelength of 488 ± 10 nm and an emission wavelength of 511 ± 10 nm.

文章：A simple method for effective cryopreservation of antlerogenic periosteum of sika deer

發(fā)表單位：長春理工大學(xué)

期刊：Journal of Experimental Zoology-A

IF值：1.9    

中科院分區(qū)：Q3

檢測類型：吸收光

檢測應(yīng)用：CCK8細(xì)胞活性

具體方法：The cells were seeded in 96‐well‐plates at density of 2000 cells/well. When the cells reached around 60% confluence, the medium was replaced by 90 μL new complete medium and 10 μL CCK‐8 solution/ well for further 2 h. Subsequently, the solution was measured at 450 nm using a microplate reader (Feyond‐A300).

文章：p38 and ERK1/2-Dependent Activation of c-Jun Is Required for the Downregulation of Oxidative Stress-Induced ERα in Hypothalamic Astrocytes

發(fā)表單位：福建醫(yī)科大學(xué)協(xié)和醫(yī)院

期刊：Neuroendocrinology

IF值：3.2    

中科院分區(qū)：Q2

檢測類型：熒光

檢測應(yīng)用：ROS

具體方法：The results are expressed by the fluorescence value, which was measured using an excitation wavelength of 480 nm and emission wavelength of 525 nm (Feyond- A300)

文章：Ilyomycin L, a novel resorcylic acid lactone with furan nucleus from Ilyonectria sp. DWS906

發(fā)表單位：中南大學(xué)湘雅醫(yī)院老年疾病國家臨床醫(yī)學(xué)研究中心藥學(xué)系

期刊：Journal of Molecular Structure

IF值：4    

中科院分區(qū)：Q2

檢測類型：吸收光

檢測應(yīng)用：CCK8細(xì)胞活性

具體方法：

Finally, 10 μL of CCK-8 was added to each well for 1–4h, and the absorbance was measured at 450 nm using a microplate

reader (Feyond-A300, allsheng, Hangzhou, China).

文章：The mechanistic insights of essential oil of Mentha piperita to control Botrytis cinerea and the prospection of lipid nanoparticles to its application

發(fā)表單位：Universidad de La Frontera

期刊：Microbiological Research

IF值：6.1    

中科院分區(qū)：Q1

檢測類型：熒光

檢測應(yīng)用：

MitoTracker? Orangepropidium iodide、Calcein-AM、CellROX Deep Red、TPA

具體方法：

Furthermore, the fluorescence intensity was determined in a multimodal microplate reader (Feyond-a300, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). The following excitation and emission wavelengths were used in this study: 554/575 nm for MitoTracker? Orange, 535/617 nm for propidium iodide, 477/520 nm for Calcein-AM, and 350/432 for Calcofluor White.

A standard curve of H2O2 (SigmaAldrich?, St. Louis, MO, USA) was measured from 1 μM to 200 μM tointerpolate de obtained optical density. The following excitation and emission wavelengths were used in this study: 645/675 nm for CellROX Deep Red, 320/425 nm for TPA.

文章：Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

發(fā)表單位：福建醫(yī)科大學(xué)第一附屬醫(yī)院

期刊：Analytical Methods

IF值：2.7    

中科院分區(qū)：Q3

檢測類型：吸收光

檢測應(yīng)用：光譜檢測

具體方法：

The UV-vis absorption spectra were recorded using a microplate reader (Feyond-A300).

The color of the detection system was recorded by a phone camera. The UV-vis absorbance spectra were measured using a microplate reader

文章：Macrogenomics-Based Analysis of the Effects of Intercropped Soybean Photosynthetic Characteristics and Nitrogen-Assimilating Enzyme Activities on Yield at Different Nitrogen Levels

發(fā)表單位：吉林大學(xué)

期刊：Microorganisms

IF值：4.1    

中科院分區(qū)：Q2

檢測類型：吸收光

檢測應(yīng)用：CCK8

具體方法：

The activity titre was determined using an enzyme label (Feyond-A300, 0–4 OD, ALLSHENG, Shanghai, China) at respective wavelengths of 340 nm, 540 nm, 340 nm, 630 nm, and 240 nm.

文章：Examination of the relationship of carbonate alkalinity stress and ammonia metabolism disorder-mediated apoptosis in the Chinese mitten crab, Eriocheir sinensis: Potential involvement of the ROS/MAPK signaling pathway

發(fā)表單位：中國科學(xué)院黑龍江水產(chǎn)研究所

期刊：Aquaculture

IF值：3.9    

中科院分區(qū)：Q1

檢測類型：熒光

檢測應(yīng)用：蛋白定量

具體方法：Finally, the fluorescence of hemocytes was measured with a fluorescence microplate reader (Feyond-A300) at an ex/em wavelength of 470/525 nm,

文章：Silver nanoparticles induce endothelial cytotoxicity through ROS-mediated mitochondria-lysosome damage and autophagy perturbation: The protective role of N-acetylcysteine

發(fā)表單位：寧夏醫(yī)科大學(xué)總醫(yī)院

期刊：Toxicology

IF值：4.8    

中科院分區(qū)：Q3

檢測類型：發(fā)光

檢測應(yīng)用：ATP

具體方法：ATP content was measured using a multifunctional microplate reader (Feyond-A300, AllSheng Instruments, Hangzhou City, Zhejiang, China), expressed as the number of relative light units (RLUs), and normalized to the protein concentration

文章：Purification, characterization, and antioxidant ability of polysaccharides from Phascolosoma esculentas

發(fā)表單位：寧德師范學(xué)院

期刊：Food Science & Nutrition

IF值：3.5    

中科院分區(qū)：Q2

檢測類型：熒光

檢測應(yīng)用：ROS

具體方法：Thereafter, C. elegans were mixed with DCFH-DA (20 μM) and then reacted for 2 h without light. Subsequently, C. elegans were transferred to a glass slide and photographed by fluorescence microscope (DM6B, Leica, Germany). Additionally, the relative fluorescence intensity was measured by an enzyme-labeled instrument (Feyond-A300, Shanghai, China).

文章：Macrocyclic trichothecenes from Myrothecium verrucaria PA 57 and their cytotoxic activity

發(fā)表單位：中南大學(xué)湘雅醫(yī)院

期刊：Chinese Journal ofNatural Medicines

IF值：4    

中科院分區(qū)：Q2

檢測類型：吸收光

檢測應(yīng)用：CCK8細(xì)胞活性

具體方法：

Finally, 10 μL of CCK-8 was added to each well for 1?4 h, and the absorbance was detected at 450 nm with a microplate reader (Feyond- A300, allsheng, Hangzhou, China). The GraphPad Prism 9.5.1 software was adopted to calculate IC50 values.

文章：Maize/Soybean Intercropping with Straw Return Increases Crop Yield by Influencing the Biological Characteristics of Soil

發(fā)表單位：吉林農(nóng)業(yè)大學(xué)

期刊：Scientific Reports

IF值：4.1    

中科院分區(qū)：Q2

檢測類型：吸收光

檢測應(yīng)用：酶活

具體方法：Their activity was determined using an enzyme-labeling instrument (Feyond- A300, 0-4 OD, Aosens, Hangzhou, China) at wavelengths of 630, 240, 508, and 600 nm, respectively

文章：NUDT21 interacts with NDUFS2 to activate the PI3K/AKT pathway and promotes pancreatic cancer pathogenesis

發(fā)表單位：首都醫(yī)科大學(xué)宣武醫(yī)院

期刊：Journal of Cancer

IF值：3.3    

中科院分區(qū)：Q3

檢測類型：吸收光

檢測應(yīng)用：NADP + /NADPH

具體方法：In particular, NADP + / NADPH ratios were determined using a NADP + /NADPH Assay kit (S0179, Beyotime) and were detected using a colorimetric assay under a microplate reader (Feyond-A400, ALLSHENG, China) with detection wavelengths of 570 nm, respectively.

文章：OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death

發(fā)表單位：首都醫(yī)科大學(xué)宣武醫(yī)院

期刊：Cell Death Discovery

IF值：6.1    

中科院分區(qū)：Q2

檢測類型：吸收光

檢測應(yīng)用：NADP + /NADPH

具體方法：In particular, NADP+/NADPH ratios were determined using a NADP+/NADPH Assay kit (S0179, Beyotime) and were detected using a colorimetric assay under a microplate reader (Feyond-A400, ALLSHENG, China) with detection wavelengths of 570 nm, respectively.